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    Structured Review

    TargetMol recombinant proteins verteporfin targetmol
    Recombinant Proteins Verteporfin Targetmol, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+proteins+verteporfin+targetmol/Verteporfin/pm32589942-256-60-63
    Average 94 stars, based on 29 article reviews
    recombinant proteins verteporfin targetmol - by Bioz Stars, 2026-09
    94/100 stars

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    Virus:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Recombinant:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    SYBR Green Assay:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    RNA Extraction:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Viability Assay:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Bradford Protein Assay:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Magnetic Cell Separation:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    RNAscope:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    Staining:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    TUNEL Assay:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..

    RNA Sequencing:

    Article Title: Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-YAP (63.7) Santa Cruz Biotechnology Cat# sc-1001199; RRID: AB_1131430 Rabbit monoclonal anti-MOB1 (E1N9D) Cell Signaling Technology Cat# 13730; RRID: AB_2783010 Rabbit monoclonal anti-pMOB1 (Thr35) (D2F10) Cell Signaling Technology Cat# 8699; RRID: AB_11139998 Mouse monoclonalanti-PP2A Catalytic alpha (Clone 46) BD Biosciences Cat# 610555; RRID: AB_397909 Rabbit polyclonal anti-pMST1 (Thr183)/MST2 (Thr180) Cell Signaling Technology Cat# 3681; RRID: AB_330269 Rabbit polyclonal anti-STK3/MST2 (pT180) + MSP/MST1 (pT183) Abcam Cat#ab79199; RRID: AB_2271183 Mouse monoclonal anti-Krs-1(87.K) Santa Cruz Biotechnology Cat# sc-130405; RRID: AB_2196475 Rabbit polyclonal anti-MST2 Cell Signaling Technology Cat# 3952; RRID: AB_2196471 Rabbit polyclonal anti-pYAP (Ser127) Cell Signaling Technology Cat# 4911S; RRID: AB_2218913 Mouse monoclonal anti-YAP (H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID: AB_10612397 Rabbit polyclonal anti-STRN3 Thermo Fisher Scientific Cat# PA5-31368; RRID: AB_2548842 Mouse monoclonal anti-b-Actin Sigma-Aldrich Cat# A2228; RRID: AB_476697 Rabbit polyclonal anti-MST1 Cell Signaling Technology Cat# 3682; RRID: AB_2144632 Rabbit polyclonal anti-Striatin Abcam Cat# ab193000 Rabbit monoclonal anti-Striatin 4 (EPR11801) Abcam Cat# ab177155 Rabbit monoclonal anti-PP2A A Subunit (81G5) Cell Signaling Technology Cat# 2041; RRID: AB_2168121 Goat polyclonal anti-PPP2R1A Novus Biologicals Cat# NB100-1497, RRID: AB_2169802 Mouse monoclonal anti-FLAG (clone M2) Sigma-Aldrich Cat# F3165; RRID: AB_259529 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID: AB_1549585 Mouse monoclonal anti-GFP (B-2) Santa Cruz Biotechnology Cat# sc-9996; RRID: AB_627695 Mouse monoclonal anti-a-SMA (1A4) Abcam Cat# ab7817, RRID: AB_262054 Rabbit polyclonal anti-ERK1 (K-23) Santa Cruz Biotechnology Cat# sc-94, RRID: AB_2140110 Rabbit polyclonal anti-Ki67 Abcam Cat# ab15580; RRID: AB_443209 Mouse monoclonal anti-CD44 v9 Cosmo Bio Cat# CAC-LKG-M001 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat# A32723; RRID: AB_2633275 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 Thermo Fisher Scientific Cat # A32849; RRID: AB_2762840 (Continued on next page) Cancer Cell 38, 1–14.e1–e9, July 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli DH5a Shenzhen KT Life technology Cat# KTSM101L Escherichia coli BL21 (DE3) Codon Plus Zhang et al., 2017 N/A Escherichia coli DH10Bac Wang et al., 2016a, 2016b N/A Biological Samples Human gastric cancer tissues and paired adjacent tissues Department of General Surgery, Hua’shan Hospital, Fudan University N/A Chemicals, Peptides, and Recombinant Proteins Verteporfin TargetMol Cat# T3112 MNNG TCI Cat# M0527 Simvastatin TargetMol Cat# T0687 Metformin TargetMol Cat# T0740 qPCR SYBR Green Master Mix (No Rox) Yeasen Cat# 11201ES03 Human ON-TARGET plus siRNA Library (Phosphatases) Dharmacon Cat# G-103705-05 RNA Isolater Total RNA Extraction Reagent Vazyme Biotech Cat# R401-01 SHAP This paper N/A Linear Peptides Sangon Biotech N/A Super-TDU Sangon Biotech N/A Critical Commercial Assays BCA Protein Assay Kit Biotechwell Cat# WB2123 Cell Titer-Luminescent cell viability assay Beyotime Cat# C0065M Bradford Protein assay kit TaKaRa Cat# T9310A MACS/Mouse Cell Depletion Kit Miltenyi Biotech Cat# 130-104-694 DAB kit Beyotime Cat# P0202 RNA scope Probe Hs-CTGF Advanced Cell Diagnostics Cat# 560581 RNA scope Probe Mm-CTGF Advanced Cell Diagnostics Cat# 314541 H&E staining kit Weiao Biotech Cat# WH2144 PAS staining kit Solarbio Cat# G1281 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062S TUNEL Apoptosis Detection Kit (Alexa Fluor 488) Yeasen Cat# 40307 Deposited Data RNA-seq This paper Accession Number GEO: GSE135085 and GSE135086 Structure of STRN3-PP2Aa This paper PDB: 6IUR Structure of B/B55-PP2A Xu et al., 2008 PDB: 3DW8 Structure of B0/B56-PP2A Xu et al., 2006 PDB: 2NPP Structure of B00/PR72-PP2A Wlodarchak et al., 2013 PDB: 4I5L Experimental Models: Cell Lines Human: HGC-27 cells Cell library, SIBCB TCHu22 Human: AGS cells Cell library, SIBCB TCHu232 Human: BGC-823 cells Cell library, SIBCB TCHu11 Human: NCI-N87 cells Cell library, SIBCB TCHu130 Human: MGC-803 cells Cell library, SIBCB TCHu84 Human: KATO-III cells Cellbank, RIKEN BRC RCB2088 Human: MKN-45 cells Cellbank, RIKEN BRC RCB1001 Human: MKN-7 cells Cellbank, RIKEN BRC RCB3687 Human: NUGC-4 cells Cellbank, RIKEN BRC RCB1939 Human: SH-10-TC cells Cellbank, RIKEN BRC RCB1940 (Continued on next page) e2 Cancer Cell 38, 1–14.e1–e9, July 13, 2020 ..



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